Preservation of Organs for Transplant Research Paper

Preservation of Organs for Transplant - Research Paper Example This paper seeks to analyze history of organ transplant, what happens to blood flow and metabolism; the length of time the tissue is available for transplant, size of the tissue in relation to efficacy of the methods of preservation, energy balance at the tissue/organ surface and how this may appropriate particular method, and the methods that are used in conjunction with hypothermia. Introduction Organ transplant is the effective therapy for end-state organ failure which primarily depends on the supply of organs of high quality and efficacy. It was developed in 1960’s based on the functional knowledge by anatomists and physiologists on the requirements of to keep body organs viable and functioning outside the body. Organ preservation on the other hand acts as a logistic aspect to enable conducting of laboratory tests and organization of other clinical activities before the actual transplant (Toledo-Pereyra, 3). Organ preservation simply calls for slowing biological deteriorat ion in organs removed from their normal physiological environment necessitating advancement of methods and approaches to achieve the process. Methods Cooling: This involves reduction of temperatures to inhibit cellular metabolism and requirements for oxygen in a bid to prevent tissue injury. The organs are preserved in a specific solution which in most cases prevent the molecular, cell and tissue changes in a bid to prevent oxidative stress and inflammation/cytokine production which would affect the conditions of the tissue. Hypothermic Machine Perfusion [HMP]: this method depends on activating residual metabolism which greatly relies on energy generation synonymous with the mammalian need for oxygen supply for aerobic metabolism through vascular perfusion. Simple Static cold storage [SCS]: It is associated with preservation for livers, lungs, pancreas or heart with a basic concept of cooling supplemented with use of special preservation solutions aimed at modifying inevitable cellu lar molecular changes. Discussion In cooling, blood flow and metabolism are suppressed so as to inhibit uncontrolled cellular/ molecular growth before the transplant. This elongates viability of the tissue/organ with respect to time by â€Å"chilling of organs† making it the widely used approach in the preservation history. The efficacy of this method depends on the size of the tissue/organ which dictates the maximum time that it can be preserved before the transplant. The energy balance is based on the mitochondrial dysfunction by disrupting its membrane permeability allowing accumulation of calcium, sodium and water within the cell which in turn inhibits uncontrolled growth/destruction of the tissue. HMP on the other hand involves a non-pulsatile blood flow coupled with low/minimal oxygen supply and low temperatures to inhibit metabolic changes of energetic substrates. Uniform myocardium cooling and constant supply of needed metabolic substances, facilitates early post-stor age capacity a method that necessitates HMP in the liver transplant. Energy balance on this method relies on the balance of oxygen supply and the aeration of perfusates and the preservation solutions (Hornick and Marlene, 3). SCS involves much of the HMP procedures but differs with the concentration of chilled solutions and

The French Revolution Essay Example | Topics and Well Written Essays - 750 words

The French Revolution - Essay Example But more interestingly, it was a rebellion against the conventional ideas and the established philosophy, with even religious discord and a struggle for supremacy (Smitha). This paper purports to discuss the main aspects of the revolution, both in terms of its reasons, and its effects. Let us consider the economic and political reasons first. France was emerging as the second world power after Britain, and there was a relentless struggle for world domination (Smitha). The French had colonies and their economic status was improving. However, their population was increasing side by side, and at a much rapid pace. Moreover, the colonies in the New World were getting independent and forming the United States of America, so the trade and commerce was divided, with Britain taking the lead (Smitha). The population of France had increased by five or six million in a span of roughly fifty years, whereas the food production was still slow and rudimentary, unsuitable to provide for the increase in the people to be fed (Smitha). In the 1780s, Paris alone had a population of 650,000 (Smitha). However, around eighty percent of the crop production around Paris was done for personal consumption by the farmers, and only around twenty percent was available in the market (Smitha). This meant that any loss in the yield, which happened frequently due to poor methods of farming and feudal system, made the common man go hungry (Smitha). This was worsened by the fact that the French economy was by large an agricultural economy, so there was no other major source of income for the government (Smitha). The government had not provided any means of storage or relief in times of need, and so the prices of bread, the staple food of the French, rose as the availability declined (Smitha). Moreover, the textile industry, which was till then a leading industry, was overtaken by the British who provided cheaper and better quality cloth to the French, thereby decreasing French export and increasi ng their import (Smitha). These, and many other economic and political factors led to a great divide between the ruling class and the common man. In 1788, there was a terrible hailstorm, which led to the worst crop yield in forty years, giving way to a most severe winter season (Smitha). Most of the people were jobless. A riot broke out (Smitha), which soon formed the shape of the revolution that went in history as the French Revolution. The intellectual and philosophical reasons behind the revolution were as important as the economic and political motives. There were two distinct groups of thinkers and socialites, the conservatives and the radicals (Cody). It is interesting to note that this intellectual revolution, for the most part, originated elsewhere outside of France, mostly in Britain, but it effected the French in the most potent manner, who rose to rebel against the established regime (Cody). There rebellion, in turn, effected the British and the rest of Europe in a profou nd way. The conservatives sided with Newton and Locke, and believed in reason, whereas, the radicals included Blake, Godwin, and Paine, among others (Cody). The French were then more influenced by the radicals who promised equality, brotherhood, and freedom (Cody). This strife led to prolonged debates on the subject of humanity and liberty between the radicals and conservatives for years (Cody). The French Revolution, however, ended in a â€Å"reign of terror† (Cody). The radicals, who had been greatly praised and supported by their British counterparts, lost control and went too far in their struggle to end any traces of the former regime (Cody). They assured that by slaughtering anyone who had any